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Insampaedok-San Extract Exerts an Immune-Enhancing Effect through NF-κB p65 Pathway Activation.
- Source :
- BioMed Research International; 9/22/2023, p1-13, 13p
- Publication Year :
- 2023
-
Abstract
- Insampaedok-san (IS) has traditionally been prescribed as a medication for cold-related symptoms in Northeast Asia, including Korea and China. In this study, we focused on elucidating the molecular mechanism underlying the immunomodulatory activity of IS water extract (ISE) in macrophages. ISE significantly enhanced the levels of nitric oxide (NO) and prostaglandin E2 (PGE<subscript>2</subscript>) by increasing the expression of inducible NO synthase and cyclooxygenase-2 (COX-2) in a dose-dependent manner. ISE, which consists of many herbs, contains a large number of active compounds whose pharmacological targets and mechanisms are complicated. Therefore, network pharmacology analysis was used to predict the potential key components, targets, and mechanisms of ISE as immunomodulators. Subsequently, the network pharmacology results were validated experimentally. Seven key components were identified through HPLC-QTOF-MS. As predicted by the network pharmacology analysis, ISE increased the mRNA expression of Tnf and Il6. Furthermore, ISE increased the phosphorylation, nuclear translocation, and transcriptional activity of the p65 subunit of the nuclear factor-κB (NF-κB) signaling pathway. In contrast, rapamycin, an NF-κB inhibitor, suppressed the ISE-induced mRNA expression of Tnf and Il6. In conclusion, ISE is an immune activator that can elevate the production of NO, PGE<subscript>2</subscript>, and proinflammatory cytokines mediated by NF-κB signaling. [ABSTRACT FROM AUTHOR]
- Subjects :
- CHINESE medicine
NF-kappa B
IN vitro studies
NONSTEROIDAL anti-inflammatory agents
HIGH performance liquid chromatography
NITRIC oxide
MACROPHAGES
PHOSPHORYLATION
RESEARCH funding
PHARMACEUTICAL chemistry
CELLULAR signal transduction
TREATMENT effectiveness
CYCLOOXYGENASE 2
PLANT extracts
MESSENGER RNA
COMMON cold
MASS spectrometry
NITRIC-oxide synthases
IMMUNOMODULATORS
DINOPROSTONE
TUMOR necrosis factors
INTERLEUKINS
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- Complementary Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 172447518
- Full Text :
- https://doi.org/10.1155/2023/5458504