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Comparative transcriptomic analysis of mammary gland tissues reveals the critical role of GPR110 in palmitic acid-stimulated milk protein and fat synthesis.
- Source :
- British Journal of Nutrition; 11/28/2023, Vol. 130 Issue 10, p1665-1677, 13p
- Publication Year :
- 2023
-
Abstract
- The G protein-coupled receptors (GPCR) sensing nutritional signals (amino acids, fatty acids, glucose, etc.) are not fully understood. In this research, we used transcriptome sequencing to analyse differentially expressed genes (DEG) in mouse mammary gland tissues at puberty, lactation and involution stages, in which eight GPCR were selected out and verified by qRT-PCR assay. It was further identified the role of GPR110-mediating nutrients including palmitic acid (PA) and methionine (Met) to improve milk synthesis using mouse mammary epithelial cell line HC11. PA but not Met affected GPR110 expression in a dose-dependent manner. GPR110 knockdown decreased milk protein and fat synthesis and cell proliferation and blocked the stimulation of PA on mechanistic target of rapamycin (mTOR) phosphorylation and sterol-regulatory element binding protein 1c (SREBP-1c) expression. In summary, these experimental results disclose DEG related to lactation and reveal that GPR110 mediates PA to activate the mTOR and SREBP-1c pathways to promote milk protein and fat synthesis. [ABSTRACT FROM AUTHOR]
- Subjects :
- METHIONINE metabolism
BIOLOGICAL models
LACTATION
PROTEINS
SEQUENCE analysis
ANIMAL experimentation
CELL receptors
PUBERTY
MTOR inhibitors
MILK proteins
GENE expression profiling
BREAST
TISSUES
CELL proliferation
DESCRIPTIVE statistics
RESEARCH funding
POLYMERASE chain reaction
EPITHELIAL cells
CELL lines
FATTY acids
MICE
PHOSPHORYLATION
Subjects
Details
- Language :
- English
- ISSN :
- 00071145
- Volume :
- 130
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- British Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 173107342
- Full Text :
- https://doi.org/10.1017/S0007114523000788