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New HPLC Method for Determination of Cefquinome in Cattle Plasma and its Application for Pharmacokinetic Study.
- Source :
- Pakistan Journal of Zoology; Oct2023, Vol. 55 Issue 5, p2199-2206, 8p
- Publication Year :
- 2023
-
Abstract
- The cefquinome samples from plasma were extracted and separated by HPLC on a reversed phase C-18 column with mobile phase of acetonitrile and formic acid (0.1%) solution-(90:10, v/v). The calibration curves were linear ranged from 0.01-5μg/mL. The limit of quantification (LOQ) and limit of detection (LOD) were 0.04 and 0.01 μg/mL. The recovery of cefquinome from plasma was 73.4%, 78.33%, and 77% for low, medium and high concentration samples. The intraday and inter day relative standard deviation were less than 15%. The Cefquinome were found to be stable for more than 3 month at -70oC (degree symbol) and more than 24 h at 4oC. The present method has been applied successfully for the pharmacokinetics study in cattle. After intramuscular administration, the mean peak serum Concentrations (C<subscript>max</subscript>) was found 2.95μg/mL and achieved after (T<subscript>max</subscript>) 1.50h. The elimination half-life (t<subscript>l/2(el)</subscript>) was 2.03±0.06h. The Mean Residence Time (MRT) was 2.58±0.05h and the area under curve from zero time to infinity (AUC<subscript>0-μž</subscript>) were 9.67±0.72μg/mL/h. The newly proposed method was shown to be simple, rapid, economical and sensitive compared to the previously reported method, and thereafter applicable for the pharmacokinetics study in animals. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00309923
- Volume :
- 55
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Pakistan Journal of Zoology
- Publication Type :
- Academic Journal
- Accession number :
- 173260903
- Full Text :
- https://doi.org/10.17582/journal.pjz/20200312100316