New HPLC Method for Determination of Cefquinome in Cattle Plasma and its Application for Pharmacokinetic Study.

The cefquinome samples from plasma were extracted and separated by HPLC on a reversed phase C-18 column with mobile phase of acetonitrile and formic acid (0.1%) solution-(90:10, v/v). The calibration curves were linear ranged from 0.01-5μg/mL. The limit of quantification (LOQ) and limit of detection (LOD) were 0.04 and 0.01 μg/mL. The recovery of cefquinome from plasma was 73.4%, 78.33%, and 77% for low, medium and high concentration samples. The intraday and inter day relative standard deviation were less than 15%. The Cefquinome were found to be stable for more than 3 month at -70oC (degree symbol) and more than 24 h at 4oC. The present method has been applied successfully for the pharmacokinetics study in cattle. After intramuscular administration, the mean peak serum Concentrations (C_max) was found 2.95μg/mL and achieved after (T_max) 1.50h. The elimination half-life (t_l/2(el)) was 2.03±0.06h. The Mean Residence Time (MRT) was 2.58±0.05h and the area under curve from zero time to infinity (AUC_0-μž) were 9.67±0.72μg/mL/h. The newly proposed method was shown to be simple, rapid, economical and sensitive compared to the previously reported method, and thereafter applicable for the pharmacokinetics study in animals. [ABSTRACT FROM AUTHOR]