Protocol to study the effects of AMPK-mTOR/PINK-Parkin dual signaling pathways on the formation of coronary heart disease showing blood stasis symptom pattern based on traditional Chinese medicine theory of "heart governing blood and vessels".

In this study, we aim to combine gene transfection techniques with the modeling methods previously employed by the research group to deeply investigate the corresponding theories of traditional Chinese medicine regarding "myocardial energy metabolism" and "aortic thrombosis". Our goal is to elucidate the biological mechanism underlying the occurrence and development of coronary heart disease with blood stasis syndrome from the perspectives of "heart and vessels" and "Qi (in traditional Chinese medicine, it refers to the most fundamental and subtle substances that constitute the human body and maintain life activities. At the same time, it also has the meaning of physiological function. In terms of traditional Chinese medicine, Qi and different words are used together to express different meanings) and blood". The research content is divided into four modules as follows: 1. establishment of an animal model of coronary heart disease with blood stasis syndrome through fibrinogen overexpression. 2. Investigation of the mitochondrial quality control system in coronary heart disease with blood stasis syndrome under fibrinogen overexpression. 3. Study of platelet autophagy in coronary heart disease with blood stasis syndrome under fibrinogen overexpression. 4. Examination of the relationship between the AMPK-mTOR pathway and metabolism in platelet autophagy of coronary heart disease with blood stasis syndrome under fibrinogen overexpression. Ninety-six Sprague Dawley rats will be randomly assigned to the following groups: control group, model group, fibrinogen group and adeno-associated virus group. All rats will undergo a 14-week model construction process, and modern molecular biology methods will be employed to evaluate the model and examine relevant research indicators. The obtained data will be analyzed according to a predefined statistical analysis plan. [ABSTRACT FROM AUTHOR]