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Danlou Recipe promotes cholesterol efflux in macrophages RAW264.7 and reverses cholesterol transport in mice with hyperlipidemia induced by P407.
- Source :
- BMC Complementary Medicine & Therapies; 12/8/2023, Vol. 23 Issue 1, p1-14, 14p
- Publication Year :
- 2023
-
Abstract
- Introduction: Liver X Receptor (LXR) agonists could attenuate the development of atherosclerosis but bring excess lipid accumulation in the liver. Danlou Recipe was believed to be a benefit for improving the lipid profile. Thus, it is unclear whether Danlou Recipe could attenuate hyperlipidemia without excess lipid accumulated in the liver of mice. This study aimed to clarify if Danlou Recipe could alleviate the progression of hyperlipidemia in mice without extra lipids accumulated in the liver. Methods: Male murine macrophage RAW264.7 cells and murine peritoneal macrophages were used for the in vitro experiments. Cellular cholesterol efflux was determined using the fluorescent cholesterol labeling method. Those genes involved in lipid metabolism were evaluated by qRT‐PCR and western blotting respectively. In vivo, a mouse model of hyperlipidemia induced by P407 was used to figure out the effect of Danlou Recipe on reverse cholesterol transport (RCT) and hyperlipidemia. Ethanol extract of Danlou tablet (EEDL) was prepared by extracting the whole powder of Danlou Prescription from ethanol, and the chemical composition was analyzed by ultra-performance liquid chromatography (UPLC). Results: EEDL inhibits the formation of RAW264.7 macrophage-derived foam cells, and promotes ABCA1/apoA1 conducted cholesterol efflux in RAW264.7 macrophages and mouse peritoneal macrophages. In the P407-induced hyperlipidemia mouse model, oral administration of EEDL can promote RCT in vivo and improve fatty liver induced by a high-fat diet. Consistent with the findings in vitro, EEDL promotes RCT by upregulating the LXR activities. Conclusion: Our results demonstrate that EEDL has the potential for targeting RCT/LXR in the treatment of lipid metabolism disorders to be developed as a safe and effective therapy. [ABSTRACT FROM AUTHOR]
- Subjects :
- LIPID metabolism
DRUG therapy for hyperlipidemia
DISEASE progression
DRUG efficacy
IN vitro studies
REVERSE transcriptase polymerase chain reaction
BIOLOGICAL models
STATISTICS
HERBAL medicine
IN vivo studies
HIGH performance liquid chromatography
CELL culture
STAINS & staining (Microscopy)
LIVER
ANIMAL experimentation
WESTERN immunoblotting
ORAL drug administration
ONE-way analysis of variance
MACROPHAGES
COMPARATIVE studies
DNA-binding proteins
RESEARCH funding
FLUORESCENT antibody technique
ENZYME-linked immunosorbent assay
DESCRIPTIVE statistics
DATA analysis
CHINESE medicine
CHOLESTEROL
MICE
DRUG administration
DRUG dosage
EVALUATION
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 23
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 174096560
- Full Text :
- https://doi.org/10.1186/s12906-023-04253-9