Back to Search
Start Over
MiR-184-5p represses neuropathic pain by regulating CCL1/CCR8 signaling interplay in the spinal cord in diabetic mice.
- Source :
- Neurological Research; Jan2024, Vol. 46 Issue 1, p54-64, 11p
- Publication Year :
- 2024
-
Abstract
- Diabetic neuropathic pain (DNP) is a serious complication for diabetic patients involving nervous system. MicroRNAs (miRNAs) are small-noncoding RNAs which are dysregulated in neuropathic pain, and might be critical molecules for pain treatment. Our previous study has shown miR-184-5p was significantly downregulated in DNP. Therefore, the mechanism of miR-184-5p in DNP was investigated in this study. A DNP model was established through streptozotocin (STZ). The pharmacological tools were injected intrathecally, and pain behavior was evaluated by paw withdrawal mechanical thresholds (PWMTs). Bioinformatics analysis, Dual-luciferase reporter assay and fluorescence-in-situ-hybridization (FISH) were used to seek and confirm the potential target genes of miR-184-5p. The expression of relative genes and proteins was analyzed by quantitative reverse transcriptase real-time PCR (qPCR) and western blotting. MiR-184-5p expression was down-regulated in spinal dorsal on days 7 and 14 after STZ, while intrathecal administration of miR-184-5p agomir attenuates neuropathic pain induced by DNP and intrathecal miR-184-5p antagomir induces pain behaviors in naïve mice. Chemokine CC motif ligand 1 (CCL1) was found to be a potential target of miR-184-5p and the protein expression of CCL1 and the mRNA expression of CCR8 were up-regulated in spinal dorsal on days 7 and 14 after STZ. The luciferase reporter assay and FISH demonstrated that CCL1 is a direct target of miR-184-5p. MiR-184-5p overexpression attenuated the expression of CCL1/CCR8 in DNP; intrathecal miR-184-5p antagomir increased the expression of CCL1/CCR8 in spinal dorsal of naïve mice. This research illustrates that miR-184-5p alleviates DNP through the inhibition of CCL1/CCR8 signaling expression. [ABSTRACT FROM AUTHOR]
- Subjects :
- NEURALGIA
SPINAL cord
GENE expression
REVERSE transcriptase
NERVOUS system
Subjects
Details
- Language :
- English
- ISSN :
- 01616412
- Volume :
- 46
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Neurological Research
- Publication Type :
- Academic Journal
- Accession number :
- 174100048
- Full Text :
- https://doi.org/10.1080/01616412.2023.2257454