Improvements on Restricted Insecticide Application Protocol for Control of Human and Animal African Trypanosomiasis in Eastern Uganda.

Background: African trypanosomes constrain livestock and human health in Sub-Saharan Africa, and aggravate poverty and hunger of these otherwise largely livestock-keeping communities. To solve this, there is need to develop and use effective and cheap tsetse control methods. To this end, we aimed at determining the smallest proportion of a cattle herd that needs to be sprayed on the legs, bellies and ears (RAP) for effective Human and Animal African Trypanosomiasis (HAT/AAT) control. Methodology/Principal finding: Cattle in 20 villages were ear-tagged and injected with two doses of diminazene diaceturate (DA) forty days apart, and randomly allocated to one of five treatment regimens namely; no treatment, 25%, 50%, 75% monthly RAP and every 3 month Albendazole drench. Cattle trypanosome re-infection rate was determined by molecular techniques. ArcMap V10.3 was used to map apparent tsetse density (FTD) from trap catches. The effect of graded RAP on incidence risk ratios and trypanosome prevalence was determined using Poisson and logistic random effect models in R and STATA V12.1 respectively. Incidence was estimated at 9.8/100 years in RAP regimens, significantly lower compared to 25.7/100 years in the non-RAP regimens (incidence rate ratio: 0.37; 95% CI: 0.22–0.65; P<0.001). Likewise, trypanosome prevalence after one year of follow up was significantly lower in RAP animals than in non-RAP animals (4% vs 15%, OR: 0.20, 95% CI: 0.08–0.44; P<0.001). Contrary to our expectation, level of protection did not increase with increasing proportion of animals treated. Conclusions/significance: Reduction in RAP coverage did not significantly affect efficacy of treatment. This is envisaged to improve RAP adaptability to low income livestock keepers but needs further evaluation in different tsetse challenge, HAT/AAT transmission rates and management systems before adopting it for routine tsetse control programs. Author Summary: Poverty, hunger and human ill-health aggravated by trypanosomiasis in Sub-Saharan Africa can only be reduced by developing and using cheap and effective tsetse control methods. To further reduce the cost of tsetse control by restricting insecticides to the legs, belly and ears (RAP) we set out to determine the lowest RAP coverage that can effectively control tsetse. Cattle in 20 south-eastern Uganda villages were randomly allocated to 5 treatment groups, ear-tagged for ease of follow-up and treated twice forty days apart with a trypanocide at the beginning of the trial. Cattle in regimens 2–4 received monthly graded RAP (25%, 50% and 75% of village herd respectively), while those in regimens 1 and 5 received no more treatment and deworming once every three months respectively. Molecular techniques were used to check for trypanosome infections, while tsetse apparent density was determined by traps at 161 locations in the district. About 25% RAP coverage was effective at controlling T. brucei s.l. while 50–75% RAP coverage would need to be used for effective T.vivax and T.congolense nagana control. Use of RAP at lower herd coverage is envisaged to reduce its cost, damage to the environment and improve its uptake in resource poor communities. [ABSTRACT FROM AUTHOR]