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Balancing Hormones and Gene Expressions for Rooting Success: Lovastatin Unveils Cytokinin Inhibition in Malus prunifolia var. ringo Apple Stem Cuttings.

Authors :
Sun, Sinuo
Tahir, Muhammad Mobeen
Xie, Zushu
Wei, Pengyan
Yu, Jianing
Liu, Hangkong
He, Yinnan
Ren, Xiaoying
Ma, Yuanyuan
Mao, Jiangping
Source :
Horticulturae; Dec2023, Vol. 9 Issue 12, p1341, 11p
Publication Year :
2023

Abstract

Adventitious root (AR) formation is the key to asexual reproduction; however, cytokinin (CK) hampers AR formation. But the mechanism by which CK inhibits it is still unknown. In this study, we used Malus prunifolia var. ringo apple stem cuttings that were treated with exogenous 6-benzyl adenine (6-BA) at 1 mg/L and lovastatin (CK biosynthesis inhibitor) at 1 mg/L to compare with control (untreated) cuttings. The results indicated that the control and 6-BA-treated cuttings failed to produce ARs; however, lovastatin-treated cuttings successfully produced a few ARs after 20 days (d) of treatments by increasing indole-3-acetic acid (IAA) and reducing zeatin riboside (ZR) content at several time points. The 6-BA treatment induced the expression of CK-related genes, such as MdARR3, MdARR5, MdARR5-2, MdAKH4, and MdCKX5, at most time points. However, lovastatin-treated cuttings reduced their expression, which favors AR formation. Furthermore, the expression of auxin-related genes, including MdIAA23, MdARF7, and MdARF19, was induced by lovastatin treatment. Like auxin-related genes, several root-development-related genes (MdWOX5, MdWOX11, MdLB29, and MdARRO1) were also promoted in response to lovastatin treatment that were repressed by 6-BA and control cuttings. In conclusion, lovastatin treatment supports AR formation by inhibiting CK biosynthesis inside the cuttings, as compared to the control and 6-BA-treated cuttings. This study laid the foundation for future studies on the relationship of CK biosynthesis inhibitors with adventitious rooting in apples and other crops. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23117524
Volume :
9
Issue :
12
Database :
Complementary Index
Journal :
Horticulturae
Publication Type :
Academic Journal
Accession number :
174439039
Full Text :
https://doi.org/10.3390/horticulturae9121341