Simultaneous determination of 20 kinds of mycotoxins in maize straw feed by QuEChERS-ultra performance liquid chromatography-triple quadrupole mass spectrometry.

The experiment was to establish an analytical method for the simultaneous determination of 20 kinds of mycotoxins in maize straw feed by QuEChERS-UPLC-MS/MS. The samples were extracted with acetonitrile-acetic acid (95 : 5), and the supernatant was purified by anhydrous MgSO₄, PSA and C18. Then an appropriate amount of supernatant was blown to dry with nitrogen. The samples were reconstituted with methanol-water-acetic acid (30 : 70 : 0.1), passed through a PTFE filter membrane, and separated on a C18 chromatographic column. Using the multiple reaction monitoring (MRM) scan mode to monitor the target compounds and the results were quantified by the external standard method. The results showed that the linear ranges of the 20 mycotoxins were good, and the correlation coefficients (R²) were greater than 0.99. The limits of detection were 0.15~3.10 µg/kg, with limits of quantification were 0.49~10.33 µg/kg. The recoveries at the three levels were within the range of 70.4%~118.3%, with relative standard deviations of 1%~10% (n=6). The study indicates that the method was easy to operate, reproducible and highly accurated and sensitive, which can be used for routine screening of mycotoxins in corn stalk feed. [ABSTRACT FROM AUTHOR]