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A novel human CD32 mAb blocks experimental immune haemolytic anaemia in Fc γRIIA transgenic mice.
- Source :
- British Journal of Haematology; Jul2005, Vol. 130 Issue 1, p130-137, 8p
- Publication Year :
- 2005
-
Abstract
- A fully human IgG1 kappa antibody (MDE-8) was generated, which recognised Fc-gamma receptor IIa (Fc γRIIa) molecules on CD32 transfectants, peripheral blood monocytes, polymorphonuclear cells and platelets. This antibody blocked Fc γRIIa ligand-binding via its F(ab′)<subscript>2</subscript> fragment. Overnight incubation of monocytes with F(ab′)<subscript>2</subscript> fragments of MDE-8 leads to a c. 60% decrease in cell surface expression of Fc γRIIa. MDE-8 whole antibody induced a concomitant c. 30% decrease of Fc γRI on THP-1 cells and monocytes. In humans Fc γRIIa plays an important role in the clearance of antibody-coated red blood cells in vivo. As an equivalent of Fc γRIIa does not exist in mice, the in vivo effect of MDE-8 was studied in an Fc γRIIa transgenic mouse model. In these mice, antibody-induced anaemia could readily be blocked by MDE-8. These data document a new human antibody that effectively blocks Fc γRIIa, induces modulation of both Fc γRIIa and Fc γRI from phagocytic cells, and ameliorates antibody-induced anaemia in vivo. [ABSTRACT FROM AUTHOR]
- Subjects :
- IMMUNOGLOBULIN G
HEMOLYTIC anemia
ANEMIA
AUTOIMMUNITY
TRANSGENIC mice
HEMATOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00071048
- Volume :
- 130
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- British Journal of Haematology
- Publication Type :
- Academic Journal
- Accession number :
- 17450359
- Full Text :
- https://doi.org/10.1111/j.1365-2141.2005.05571.x