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Establishment of a homogeneous chemiluminescence immunoassay for cat dander sIgE antibody detection.
- Source :
- Journal of Asthma; Jan2024, Vol. 61 Issue 1, p10-19, 10p
- Publication Year :
- 2024
-
Abstract
- In vitro specific IgE (sIgE) testing has become an important tool for the diagnosis of IgE-mediated allergic diseases. Current methods used to detect allergen sIgE are time consuming and/or expensive. Therefore, a new method was developed for rapid quantitative detection of cat dander-sIgE antibody based on homogeneous chemiluminescence immunoassay. Selection of chemibeads with different chemical groups, and the best Light-initiated chemiluminescence assay (LiCA) analytical mode for cat dander-sIgE detection. To validate and eliminate the interference of IgE on the detection of cat dander-sIgE, concentration of biotinylated anti-human IgE antibody was optimized. For quantification of cat dander-sIgE, a calibration curve was established, and the performance of the assay was evaluated according to clinical guidelines. Indirect LiCA is the best mode of analysis and biotinylated anti-human IgE antibody at a dilution ratio of 1:250 minimizes IgE interference. The coefficient of variation of the developed LiCA was 1.49% to 4.66%, with an intermediate precision of 6.90% to 8.21%. The LoB, LoD, and LoQ of the assay were 0.023 kUA/L, 0.056 kUA/L and 0.185 kUA/L. The coefficient of correlation (r) between LiCA and ImmounoCAP was 0.9478. A cat dander-sIgE quantitation assay based on homogeneous chemiluminescence immunoassay was established, which could be a new reliable analytical tool for the determination of cat dander-sIgE. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02770903
- Volume :
- 61
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Asthma
- Publication Type :
- Academic Journal
- Accession number :
- 174521296
- Full Text :
- https://doi.org/10.1080/02770903.2023.2234994