Effects of Prunus cerasifera Ehrh. polyphenol extract on glucose metabolism in insulin-resistant HepG2 cells based on AMPK signaling pathway.

AIM: To investigate the effects of Prunus cerasifera Ehrh. polyphenol extract (PCE) on glucose metabolism in insulin-resistant HepG2 (lR-HepG2) cells. METHODS: An IR-HepG2 cells model was established using high sugar and high insulin induction; a control group, an insulin resistance (IR) model group, a PCE treatment group with different concentrations, and a metformin treatment group were set up. Cells were treated with 50, 100, 200, and 400 mg/L of polyphenol extract for 24 hours, respectively, to detect their effects on cellular glucose consumption. At the same time, use thiazole blue (MTT) colorimetry to evaluate cell viability. Real-time PCR and Western blot were used to detect expression of glucose metabolism-related mRNA and proteins. RESULTS: Prunus cerasifera Ehrh. polyphenol extract can significantly increase glucose consumption in IR-HepG2 cells, and cell activity tends to increase and then decline as its concentration increases; At mRNA and protein levels, in addition to significantly increasing the expression of phosphorylated adenosine monophosphate activated protein kinase (p-AMPK) protein in IR-HepG2 cells, it also decrease glycogen synthase kinase 3β (GSK3p) in glycogen synthesis and gluconeogenesis pathways, forked protein O1 (FoxOl), peroxisome proliferator activates gamma co-receptor activation 1-alpha (PGC-la), glucose-6-phosphatase (G6Pase) and phosphoenol pyruvate carboxykinase (PEPCK) expression. CONCLUSION: Prunus cerasifera Ehrh. polyphenol extract can activate the AMPK signaling pathway in IR-HepG2 cells, to further inhibit gluconeogenesis and glycogen synthesis pathways, thereby improving the insulin resistance state of HepG2 cells. [ABSTRACT FROM AUTHOR]