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No-ozone cold plasma induces apoptosis in human neuroblastoma cell line via increased intracellular reactive oxygen species (ROS).
- Source :
- BMC Complementary Medicine & Therapies; 1/20/2024, Vol. 24 Issue 1, p1-15, 15p
- Publication Year :
- 2024
-
Abstract
- Background: This study aimed to evaluate the effect of argon-based No-ozone Cold Plasma (NCP) on neuroblastoma cancer cell apoptosis. Methods: Experiments were performed with SK-N-SH and HS 68. Cell cultures were treated with NCP for 1, 3, and 5 min. NCP was applied using three different strategies: direct NCP application to cell cultures, to only media, and to only cells. Evaluation of cell viability and the level of the reactive oxygen species (ROS) was performed. N-acetyl-L-cysteine (NAC) was also used to antagonize intracellular ROS. Cleaved caspase 3, PARP, aquaporin (AQP) 3 and 8 were detected. Results: NCP induced a gradual decrease in the SK-N-SH cell viability. In contrast, the viability of HS 68 cells did not change. SK-N-SH cells viability was reduced the most when the only media-NCP application strategy was employed. Intracellular ROS levels were significantly increased with time. Cleaved caspase 3 and PARP were increased at 6 h after NCP application. SK-N-SH cells remained viable with NAC after NCP application. AQP 3 and 8 were over-expressed in SK-N-SH cells. Conclusion: These findings demonstrate the anti-cancer effect of NCP on neuroblastoma cells. NCP enhanced the selective apoptosis of neuroblastoma cells due to the increased intracellular ROS. [ABSTRACT FROM AUTHOR]
- Subjects :
- COLORIMETRY equipment
ANALYSIS of hydrogen peroxide
THERAPEUTIC use of antineoplastic agents
ACETYLCYSTEINE
STATISTICS
IN vitro studies
NOBLE gases
NEUROBLASTOMA
CELL culture
STAINS & staining (Microscopy)
ANALYSIS of variance
CULTURE media (Biology)
ONE-way analysis of variance
WESTERN immunoblotting
APOPTOSIS
CELL survival
T-test (Statistics)
PLASMA gases
TRANSFERASES
PHOTOGRAPHY
DESCRIPTIVE statistics
STATISTICAL hypothesis testing
RESEARCH funding
CELL lines
REACTIVE oxygen species
MEMBRANE proteins
BIOLOGICAL assay
DATA analysis software
DATA analysis
CASPASES
HYDROGEN peroxide
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 24
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 174919392
- Full Text :
- https://doi.org/10.1186/s12906-023-04313-0