Pelagibacterium flavum sp. nov., Isolated from Soil Sample.

A Gram-stain-negative, yellow, moist and circular, aerobic, motile, and rod-shaped bacterium, designated YIM 151497^T, was isolated from soil sample collected from Blue-Bridge, Weizhou Island, Guangxi province, China. Classification using a polyphasic approach suggested that strain YIM 151497^T belonged to the genus Pelagibacterium, and was closely relevant to Pelagibacterium nitratireducens JLT2005^T (98.8%), Pelagibacterium halotolerans CGMCC 1.7692^T (98.7%), Pelagibacterium lixinzhangensis H64^T (98.1%), and Pelagibacterium luteolum CGMCC 1.10267^T (97.1%). The growth ranges of temperature, pH, and NaCl were 4–40 ℃, pH 4.0–10.0, and 0–7% NaCl, respectively. It was positive for catalase and oxidase. The primary respiratory quinone was Q-10. The elemental fatty acids were Summed Feature 8 (constituting C_18:1ω7c and/or C_18:1ω6c), C_19:0 cyclo ω8c, C_16:0, and C_18:1ω7c 11-methyl. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and three unidentified glycolipids. The DNA G+C content based on the complete genome sequence was 60.7 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between strain YIM 151497^T and species of Pelagibacterium were in the ranges of 73.9–86.3% and 19.7–31.3%, respectively. The Average Amino Acid Identity (AAI) between strain YIM 151497^T and species of Pelagibacterium were in the ranges of 68.8–88.8%. On the basis of these data, strain YIM 151497^T is considered to represent a novel species of the genus Pelagibacterium with the name of Pelagibacterium flavum sp. nov. Type strain is strain YIM 151497^T (= KCTC 49826^T = CGMCC 1.61521^T = MCCC 1K08053^T). [ABSTRACT FROM AUTHOR]