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Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells.
- Source :
- Biomedicines; Jan2024, Vol. 12 Issue 1, p119, 33p
- Publication Year :
- 2024
-
Abstract
- T lymphocytes represent a promising target for genome editing. They are primarily modified to recognize and kill tumor cells or to withstand HIV infection. In most studies, T cell genome editing is performed using the CRISPR/Cas technology. Although this technology is easily programmable and widely accessible, its efficiency of T cell genome editing was initially low. Several crucial improvements were made in the components of the CRISPR/Cas technology and their delivery methods, as well as in the culturing conditions of T cells, before a reasonable editing level suitable for clinical applications was achieved. In this review, we summarize and describe the aforementioned parameters that affect human T cell editing efficiency using the CRISPR/Cas technology, with a special focus on gene knock-in. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
GENOME editing
HIV infections
CRISPRS
GENE therapy
Subjects
Details
- Language :
- English
- ISSN :
- 22279059
- Volume :
- 12
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Biomedicines
- Publication Type :
- Academic Journal
- Accession number :
- 175047697
- Full Text :
- https://doi.org/10.3390/biomedicines12010119