The transmembrane protein LbRSG from the recretohalophyte Limonium bicolor enhances salt gland development and salt tolerance.

SUMMARY: Salt glands are the unique epidermal structures present in recretohalophytes, plants that actively excrete excess Na+ by salt secretory structures to avoid salt damage. Here, we describe a transmembrane protein that localizes to the plasma membrane of the recretohalophyte Limonium bicolor. As virus‐induced gene silencing of the corresponding gene LbRSG in L. bicolor decreased the number of salt glands, we named the gene Reduced Salt Gland. We detected LbRSG transcripts in salt glands by in situ hybridization and transient transformation. Overexpression and silencing of LbRSG in L. bicolor pointed to a positive role in salt gland development and salt secretion by interacting with Lb3G16832. Heterologous LbRSG expression in Arabidopsis enhanced salt tolerance during germination and the seedling stage by alleviating NaCl‐induced ion stress and osmotic stress after replacing or deleting the (highly) negatively charged region of extramembranous loop. After screened by immunoprecipitation‐mass spectrometry and verified using yeast two‐hybrid, PGK1 and BGLU18 were proposed to interact with LbRSG to strengthen salt tolerance. Therefore, we identified (highly) negatively charged regions in the extramembrane loop that may play an essential role in salt tolerance, offering hints about LbRSG function and its potential to confer salt resistance. Significance Statement: LbRSG (Reduced Salt Gland) was characterized as a transmembrane protein participating in salt gland development and salt resistance of recretohalophyte Limonium bicolor by interacting with Lb3G16832, and the negatively charged regions in the extramembranous loop were verified to play an essential role in salt tolerance of transgenic Arabidopsis. This study facilitated research on the molecular mechanisms of salt gland development and the design of salt‐tolerant crops for agricultural production in saline soils. [ABSTRACT FROM AUTHOR]