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Brief research report: in-depth immunophenotyping reveals stability of CD19 CAR T-cells over time.
- Source :
- Frontiers in Immunology; 2024, p1-9, 9p
- Publication Year :
- 2024
-
Abstract
- Variability or stability might have an impact on treatment success and toxicity of CD19 CAR T-cells. We conducted a prospective observational study of 12 patients treated with Tisagenlecleucel for CD19<superscript>+</superscript> B-cell malignancies. Using a 31-color spectral flow cytometry panel, we analyzed differentiation stages and exhaustion markers of CAR T-cell subsets prior to CAR T-cell infusion and longitudinally during 6 months of follow-up. The majority of activation markers on CAR T-cells showed stable expression patterns over time and were not associated with response to therapy or toxicity. Unsupervised cluster analysis revealed an immune signature of CAR T-cell products associated with the development of immune cell-associated neurotoxicity syndrome. Warranting validation in an independent patient cohort, in-depth phenotyping of CAR T-cell products as well as longitudinal monitoring post cell transfer might become a valuable tool to increase efficacy and safety of CAR T-cell therapy. [ABSTRACT FROM AUTHOR]
- Subjects :
- CD19 antigen
T cells
IMMUNOPHENOTYPING
DIFFUSE large B-cell lymphomas
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Database :
- Complementary Index
- Journal :
- Frontiers in Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 175319512
- Full Text :
- https://doi.org/10.3389/fimmu.2024.1298598