Data on Biology Described by a Researcher at Kanazawa University (An Efficient Method for Isolating and Purifying Nuclei from Mice Brain for Single-Molecule Imaging Using High-Speed Atomic Force Microscopy).

Researchers at Kanazawa University in Japan have developed a new method for isolating and purifying nuclei from the mouse brain for single-molecule imaging using high-speed atomic force microscopy (HS-AFM). The study addresses the limitations of traditional optical imaging techniques, which are unable to achieve the required resolution for observing organelles and proteins within cells. The researchers utilized the rapid strainer microfiltration (RSM) protocol to prepare high-quality nuclei from the mouse brain and then used HS-AFM to study the spatiotemporal dynamics of nuclear pores. This research provides valuable insights into the dynamics of nuclear pores and their function in controlling the movement of molecules between the cell nucleus and cytoplasm. [Extracted from the article]