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Total flavonoids of Astragalus protects glomerular filtration barrier in diabetic kidney disease.
- Source :
- Chinese Medicine; 2/16/2024, Vol. 19 Issue 1, p1-13, 13p
- Publication Year :
- 2024
-
Abstract
- Background: Diabetic kidney disease (DKD) is a prevalent complication of diabetes and the leading cause of end-stage renal disease. Recent evidence suggests that total flavonoids of Astragalus (TFA) has promising effects on diabetes; however, its influence on DKD and the underlying mechanism remains unclear. Methods: In this study, we induced the DKD model using streptozotocin (STZ) in male C57BL/6J mice and utilized glomerular endothelial cell (GEC) lines for in vitro investigations. We constructed a network pharmacology analysis to understand the mechanism of TFA in DKD. The mechanism of TFA action on DKD was investigated through Western blot analysis and multi-immunological methods. Results: Our findings revealed that TFA significantly reduced levels of urinary albumin (ALB). Network pharmacology and intracellular pathway experiments indicated the crucial involvement of the PI3K/AKT signaling pathway in mediating these effects. In vitro experiments showed that TFA can preserve the integrity of the glomerular filtration barrier by inhibiting the expression of inflammatory factors TNF-alpha and IL-8, reducing oxidative stress. Conclusion: Our findings demonstrated that TFA can ameliorates the progression of DKD by ameliorating renal fibrosis and preserving the integrity of the kidney filtration barrier. These results provide pharmacological evidence supporting the use of TFA in the treatment of kidney diseases. [ABSTRACT FROM AUTHOR]
- Subjects :
- TREATMENT of diabetes
GLOMERULAR filtration rate
IN vitro studies
ALBUMINS
INTERLEUKINS
FLAVONOIDS
ANIMAL experimentation
WESTERN immunoblotting
ANTI-inflammatory agents
AMINOGLYCOSIDES
TREATMENT effectiveness
RATS
CELLULAR signal transduction
OXIDATIVE stress
RESEARCH funding
TRANSFERASES
PROTEINURIA
ASTRAGALUS (Plants)
PHARMACEUTICAL chemistry
DIABETIC nephropathies
Subjects
Details
- Language :
- English
- ISSN :
- 17498546
- Volume :
- 19
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 175528007
- Full Text :
- https://doi.org/10.1186/s13020-024-00903-3