Research Progress on Effect-Based Trigger Values for in vitro Bioassays of Environmental Water Samples.

In vitrobioassays can directly obtain the toxicity information of numerous coexisting pollutants in water environment, and has become one of the important means for toxic effect evaluations and diagnosis. However, due to the lack of toxicity effect limit standards for determining the water quality, it is difficult to use bioassays for water quality management. Therefore, more and more research is focusing on deriving the effect-based trigger values (EBTs) of in vitrobioassays. This paper reviews the establishment background and derivation principles of EBTs, and summarizes a variety of derivation methods of EBTs. At present, the methods of derivation of EBTs are divided into two categories according to the protection objectives, one is the health protection objective and the other is the ecological protection objective. EBTs for drinking water are based on the premise of protecting human health and are based on allowable daily intake (ADI), in vivo safe concentration, daily dose of 10% increase in cancer inci- dence (TD10 ) and guideline values (GV) in environmental quality standard (EQS). While, EBTs for surface water, aiming at ecological protection, were derived based on GVs values in the EQS and hazardous concentrations at the 5th percentile (HC5 ) of the species sensitivity distribution (SSD). Derivation of EBTs from GV and HC5 are two widely used frameworks. The former is dedicated to reading EBT values from GV of EQS and trying to establish derivation methods that can be widely applied to various toxicity endpoints. The latter is to obtain HC5 values of positive compounds from the species sensitivity curve. At the same time, combined with the background parameters of the water body to be measured, the EBT value of the protection ecosystem was derived. This paper compares the EBT values and characteristics derived from different methods for in vitro bioassays and summarizes the applica- tion of EBTs in water environments in order to provide theoretical basis and technical support for high-throughput in vitrobioassays for water quality assessment. [ABSTRACT FROM AUTHOR]