Single fiber curvature for muscle impairment assessment: Phase contrast imaging of stroke‐induced animals.

There are technical challenges in imaging studies that can three‐dimensionally (3D) analyze a single fiber (SF) to observe the functionality of the entire muscle after stroke. This study proposes a 3D assessment technique that only segments the SF of the right stroke‐induced soleus muscle of a gerbil using synchrotron radiation x‐ray microcomputed tomography (SR‐μCT), which is capable of muscle structure analysis. Curvature damage in the SF of the left soleus muscle (impaired) progressed at 7‐day intervals after the stroke in the control; particularly on the 7 days (1 week) and 14 days (2 weeks), as observed through visualization analysis. At 2 weeks, the SF volume was significantly reduced in the control impaired group (p =.033), and was significantly less than that in the non‐impaired group (p =.009). We expect that animal post‐stroke studies will improve the basic field of rehabilitation therapy by diagnosing the degree of SF curvature. Research Highlights: Muscle evaluation after ischemic stroke using synchrotron radiation x‐ray microcomputed tomography (SR‐μCT).Curvature is measured by segmenting a single fiber (SF) in the muscle.Structural changes in the SF of impaired gerbils at 7‐day intervals were assessed. [ABSTRACT FROM AUTHOR]