Direct Enantiomeric Separation of Indapamide by Thin Layer Chromatography using βcyclodextrin as Chiral Selector.

A novel economic thin layer chromatographic method for stereoselective separation of racemic mixture of (RS)-Indapamide and determination of their enantiomers was done. The method was based on using normal TLC plates and cyclodextrin with hydroxylic part, was used as the chiral selector. Cyclodextrin was used as an additive in silica gel to prepare a TLC plate which was a non-covalent bonding, and there was no chiral substance added in the solvent system. The mobile phase was toluene-ethyl acetate-MeOH-glacial AcOH (6:4:1:0.1). Cyclodextrin was also added to mobile phase, and there was no chiral selector in the stationary phase. The mobile phase was a mixture of toluene-ethyl acetate-MeOH-glacial AcOH-5mM CD (6:4:1:0.1:1.0). The spots were then isolated and identified. The impact of the components of mobile phase, temperature, and pH was studied for the finding the best separation conditions. The spots were sited in a chamber which had Iodine granules. [ABSTRACT FROM AUTHOR]