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Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-87 MG cell line.
- Source :
- Avicenna Journal of Phytomedicine; Mar/Apr2024, Vol. 14 Issue 2, p166-176, 11p
- Publication Year :
- 2024
-
Abstract
- Objective: Silibinin has exhibited antitumor activities. However, there are few reports about the immunomodulatory properties of silibinin on T lymphocyte function in the tumor microenvironment. Here, we determined the effects of silibinin on T cells of peripheral blood mononuclear cells (PBMCs), cultivated alone or with a human cell line of glioblastoma (U-87 MG). Materials and Methods: The proliferation of T lymphocytes was assessed by MTT test in the presence of silibinin (15 and 45 µM). Also, total antioxidant capacity (TAC), the activity of superoxide dismutase-3 (SOD3), and the levels of two cytokines interferon gamma (IFN-γ) and tumor growth beta (TGF-β) were compared between treated and untreated PBMCs alone or co-cultured with U-87 cells. Results: According to our results, silibinin raised the TAC levels and SOD3 activity in the PBMCs and in the co-culture condition. Moreover, silibinin-treated PBMCs showed higher IFN-γ levels and lower TGF-β levels. Interestingly, silibinin protected PBMCs against the U-87-induced suppression. Conclusion: Altogether, these results proposed the immunomodulatory potential of silibinin on T cells of PBMCs, as well as its partially protective effects on PBMCs against the suppression induced by U-87 MG cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- MONONUCLEAR leukocytes
T cells
SILIBININ
CELL physiology
CELL lines
OXIDANT status
Subjects
Details
- Language :
- English
- ISSN :
- 22287930
- Volume :
- 14
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Avicenna Journal of Phytomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 176113789
- Full Text :
- https://doi.org/10.22038/AJP.2023.22935