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Hydroethanolic extract of Piliostigma thonningii leaves extenuates the severity of diarrhoea in female Wistar rats.
- Source :
- Journal of Complementary & Integrative Medicine; Mar2024, Vol. 21 Issue 1, p26-37, 12p
- Publication Year :
- 2024
-
Abstract
- Nigeria ranks second in the global prevalence of diarrhoea with most cases concentrated in the Northern-region of the country. This research explored the antidiarrhoeal efficacy of the hydroethanolic extract of Piliostigma thonningii leaves (HEPTL), locally used to manage diarrhoeal conditions in Kebbi State, Nigeria. P. thonningii leaves were screened for their secondary metabolites and mineral constituents. Using 3 standard-diarrhoea models, female Wistar rats completely-randomised into six-groups of six animals each were utilised for probing the antidiarrhoeal activity of HEPTL. Animals in groups I and II served as the negative and positive controls, whereas the rats in groups III, IV and V respectively received 50, 100, and 200 mg/kg body weight-(bw) of HEPTL. Six secondary metabolites and eight minerals were found in the extract, with flavonoids and calcium being the most abundant while steroids and zinc were the least prevalent, respectively. High performance liquid chromatographic analysis revealed the presence of 19 bioactive substances. Furthermore, there was a significant (p<0.05) and dose-related reduction in diarrhoea onset, water content, and wet faeces count. Similarly, the amount of intestinal fluid and average distance traversed by the charcoal-meal were decreased dose-dependently by the HEPTL with a commensurate rise in the suppression of intestinal fluid accrual and peristalsis. Acetylcholinesterase, Na<superscript>+</superscript>/K<superscript>+</superscript>-ATPase, reduced glutathione, intestinal-alkaline phosphatase and protein levels increased significantly (p<0.05) whereas superoxide-dismutase, catalase, intestinal-nitric oxide and malondialdehyde levels all fell significantly (p<0.05). However, the level of intestinal glucose was not significantly altered. Overall, the HEPTL exhibited a profound effect in the alleviation of the severity of diarrhoea, notably at 200 mg/kg bw. [ABSTRACT FROM AUTHOR]
- Subjects :
- DIARRHEA prevention
DIARRHEA
BIOLOGICAL models
STEROIDS
HIGH performance liquid chromatography
GLUTATHIONE
SUPEROXIDE dismutase
ANTIDIARRHEALS
FECES
GASTROINTESTINAL motility
NITRIC oxide
STATISTICAL sampling
FLAVONOIDS
SEVERITY of illness index
ZINC
ALKALINE phosphatase
CATALASE
PLANT extracts
RATS
METABOLITES
CALCIUM
DRUG efficacy
MOLECULAR structure
ANIMAL experimentation
LEAVES
MINERALS
ORGANIC compounds
CHARCOAL
ACETYLCHOLINESTERASE
MALONDIALDEHYDE
EVALUATION
Subjects
Details
- Language :
- English
- ISSN :
- 15533840
- Volume :
- 21
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Complementary & Integrative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 176147914
- Full Text :
- https://doi.org/10.1515/jcim-2023-0205