Determination of tylosin tartrate by resonance light scattering spectrometry with malachite green dual wavelength superposition.

The paper aims to develop a method to rapidly detect the content of tylosin tartrate. In Clark-Lubs buffer solution with pH 6.6, malachite green can induce strong resonance light scattering due to the construction of supermolecule aggregates, and tylosin tartrate can react with malachite green cations, resulting in the decomposition of supermolecule aggregates constructed by malachite green, which significantly weakens the resonance light scattering intensity of the system. The maximum detection wavelengths are at 285 nm and 332 nm. Due to the additive nature of the resonance light intensity ( I), the two wavelengths are superimposed for measurement. The linear range of tylosin tartrate is 0.2-0.6 mg/L, the linear regression equation is IRLS= 5 299 C-766.4, the correlation coefficient ( R) is 0.999 8, and the detection limit is 0.004 1 mg/L. The result indicates that the method is simple and sensitive, and can be successfully applied for the determination of tylosin tartrate. [ABSTRACT FROM AUTHOR]