Study on pharmacodynamics and mechanism of nano-Kuiyangye in treating radiation esophagitis in rats.

Objective: To explore the curative effect of nano-Kuiyangye on radiation esophagitis in Wistar rats, and to explore its possible mechanism. Methods: Wistar rats were irradiated locally with 30Gy rays to establish an animal model of radiation esophagitis in rats. After irradiation, nano-Kuiyangye, traditional Chinese medicine ulcer solution, Kangfuxin solution, nano-hydrotalcite matrix, and distilled water were used to intervene continuously for 7 d, during which the body weight and food intake of the rats were recorded. On day 7, blood was collected from the abdominal aorta under anesthesia, and the serum was obtained by centrifugation. The expression levels of pain-related factors prostaglandin-2 (PGE-2), substance P (SP), and calcitonin gene-related peptide (CGRP) were detected by ELISA. Rats were sacrificed after blood collection, and full-length esophageal tissues were taken. Hematoxylin-eosin (HE) staining was performed to analyze the pathological changes of the rat esophagus, and Western Blotting was used to detect the expression of nuclear factor-κB (NF-κB) inflammatory protein. Results: Compared with the control group, the total food intake and body weight of the rats within 7 d after modeling were significantly decreased (P<0.05), and the expressions of pain-related factors PGE-2, SP, and CGRP were higher than those of the control group (P<0.05)., the esophageal pathological damage score increased (P<0.05), and the expression of NF-κB inflammatory protein increased (P<0.05); after treatment, the total food intake of the rats in the nano-Kuiyangye intervention group within 7 days after modeling was higher than that in other groups (P<0.05), the expressions of pain-related factors PGE-2, SP, and CGRP were lower than those in the model group (P<0.05), the esophageal pathological damage score was lower (P<0.05), and the expression of NF-κB inflammatory proteins was lower (P<0.05). Conclusion: Nano-Kuiyangye increases the food intake of rats with radiation esophagitis, reduces esophageal tissue damage, and reduces the concentration of serum pain factors; the anti-inflammatory effect of nano-Kuiyangye may be related to the reduction of NF-κB inflammatory factor levels. [ABSTRACT FROM AUTHOR]