Findings from University of Wah Update Knowledge of Citrobacter (Optimization and Production of Highly Stable Amylase Isolated From Citrobacter Portucalensis Using Low-cost Agro-industrial Wastes As Substrates: Prospective...).

Researchers from the University of Wah in Pakistan have isolated a potent thermostable bacterial amylase (A-4) from agricultural soil samples. The amylase-producing bacterial strain was identified as Citrobacter portucalensis. The researchers used low-cost agro-industrial wastes such as wheat bran, potato peel, and sugarcane bagasse as substrates for amylase production. The optimized culture conditions resulted in high amylase activity, and protein purification yielded a 4-fold increase in yield and a specific activity of 40 U/mL/min. The findings suggest that C. portucalensis (A-4) could be a promising candidate for the detergent and pharmaceutical industries. [Extracted from the article]