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Alkyne-tagged SERS nanoprobe for understanding Cu+ and Cu2+ conversion in cuproptosis processes.
- Source :
- Nature Communications; 4/15/2024, Vol. 15 Issue 1, p1-13, 13p
- Publication Year :
- 2024
-
Abstract
- Simultaneously quantifying mitochondrial Cu<superscript>+</superscript> and Cu<superscript>2+</superscript> levels is crucial for evaluating the molecular mechanisms of copper accumulation-involved pathological processes. Here, a series of molecules containing various diacetylene derivatives as Raman reporters are designed and synthesized, and the alkyne-tagged SERS probe is created for determination Cu<superscript>+</superscript> and Cu<superscript>2+</superscript> with high selectivity and sensitivity. The developed SERS probe generates well-separated distinguishable Raman fingerprint peaks with built-in corrections in the cellular silent region, resulting in accurate quantification of Cu<superscript>+</superscript> and Cu<superscript>2+</superscript>. The present probe demonstrates high tempo-spatial resolution for real-time imaging and simultaneously quantifying mitochondrial Cu<superscript>+</superscript> and Cu<superscript>2+</superscript> with long-term stability benefiting from the probe assembly with designed Au-C≡C groups. Using this powerful tool, it is found that mitochondrial Cu<superscript>+</superscript> and Cu<superscript>2+</superscript> increase during ischemia are associated with breakdown of proteins containing copper as well as conversion of Cu<superscript>+</superscript> and Cu<superscript>2+</superscript>. Meanwhile, we observe that parts of Cu<superscript>+</superscript> and Cu<superscript>2+</superscript> are transported out of neurons by ATPase. More importantly, cuproptosis in neurons is found including the oxidative stress process caused by the conversion of Cu<superscript>+</superscript> to Cu<superscript>2+</superscript>, which dominates at the early stage (<9 h), and subsequent proteotoxic stress. Both oxidative and proteotoxic stresses contribute to neuronal death. Simultaneously quantifying mitochondrial Cu<superscript>+</superscript> and Cu<superscript>2+</superscript> levels is vital for understanding the molecular mechanism of mitochondria-related biological events. Here the authors report an alkynyl-labeled SERS probe to simultaneously monitor free Cu<superscript>+</superscript> and Cu<superscript>2+</superscript> in mitochondria, and unveil their roles during ischemia and cuproptosis processes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 15
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Nature Communications
- Publication Type :
- Academic Journal
- Accession number :
- 176627184
- Full Text :
- https://doi.org/10.1038/s41467-024-47549-1