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Heterologous biosynthesis of prenylated resveratrol through multiplex metabolic engineering in Escherichia coli.
- Source :
- Green Chemistry; 4/21/2024, Vol. 26 Issue 8, p4792-4802, 11p
- Publication Year :
- 2024
-
Abstract
- Prenylated stilbenoids possess a range of biological activities, including anti-inflammatory, antioxidant, antidiabetic, anticancer, neuroprotective and vasorelaxant properties. However, their potential is limited by the scarcity of these compounds in nature. In this study, we present an eco-friendly biosynthetic method for producing prenylated resveratrol in Escherichia coli. By co-expressing the MVA pathway for DMAPP synthesis with a prenyltransferase, we successfully achieved in vivo prenylation of resveratrol. We then reconstructed a novel biosynthetic pathway for 2-C-prenyl resveratrol by integrating the resveratrol pathway. Through careful metabolic flux engineering to accumulate acetyl-CoA and malonyl-CoA, we created a high-yield strain that produced 2-C-prenyl resveratrol, achieving a significant titer of 68.4 mg L<superscript>−1</superscript> in flask cultures. This research marks the first microbial production of prenylated stilbenoids, providing a foundation for sustainable green production of these compounds. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14639262
- Volume :
- 26
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Green Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 176761540
- Full Text :
- https://doi.org/10.1039/d4gc00053f