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Ginsenoside Rd Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Inflammation and Apoptosis through PI3K/Akt Signaling Pathway.
- Source :
- American Journal of Chinese Medicine; 2024, Vol. 52 Issue 2, p433-451, 19p
- Publication Year :
- 2024
-
Abstract
- Myocardial ischemia/reperfusion (I/R) injury is the leading cause of death worldwide. Ginsenoside Rd (GRd) has cardioprotective properties but its efficacy and mechanism of action in myocardial I/R injury have not been clarified. This study investigated GRd as a potent therapeutic agent for myocardial I/R injury. Oxygen-glucose deprivation and reperfusion (OGD/R) and left anterior descending (LAD) coronary artery ligation were used to establish a myocardial I/R injury model in vitro and in vivo. In vivo, GRd significantly reduced the myocardial infarct size and markers of myocardial injury and improved the cardiac function in myocardial I/R injury mice. In vitro, GRd enhanced cell viability and protected the H9c2 rat cardiomyoblast cell line from OGD-induced injury GRd. The network pharmacology analysis predicted 48 potential targets of GRd for the treatment of myocardial I/R injury. GO and KEGG enrichment analysis indicated that the cardioprotective effects of GRd were closely related to inflammation and apoptosis mediated by the PI3K/Akt signaling pathway. Furthermore, GRd alleviated inflammation and cardiomyocyte apoptosis in vivo and inhibited OGD/R-induced apoptosis and inflammation in cardiomyocytes. GRd also increased PI3K and Akt phosphorylation, suggesting activation of the PI3K/Akt pathway, whereas LY294002, a PI3K inhibitor, blocked the GRd-induced inhibition of OGD/R-induced apoptosis and inflammation in H9c2 cells. The therapeutic effect of GRd in vivo and in vitro against myocardial I/R injury was primarily dependent on PI3K/Akt pathway activation to inhibit inflammation and cardiomyocyte apoptosis. This study provides new evidence for the use of GRd as a cardiovascular drug. [ABSTRACT FROM AUTHOR]
- Subjects :
- INFLAMMATION prevention
IN vitro studies
MYOCARDIAL ischemia
MYOCARDIAL reperfusion complications
INTRAPERITONEAL injections
PENTOBARBITAL
STATISTICAL significance
RESEARCH funding
APOPTOSIS
PHARMACEUTICAL chemistry
ENZYME-linked immunosorbent assay
CELLULAR signal transduction
IN vivo studies
REVERSE transcriptase polymerase chain reaction
PLANT extracts
MICE
CELL culture
IMMUNOHISTOCHEMISTRY
GLYCOSIDES
DRUG efficacy
MYOCARDIUM
MOLECULAR structure
WESTERN immunoblotting
ONE-way analysis of variance
GINSENG
PHOSPHOTRANSFERASES
STAINS & staining (Microscopy)
CELL survival
HEALTH outcome assessment
COMPARATIVE studies
TIME
ECHOCARDIOGRAPHY
HEART cells
TUMOR necrosis factors
Subjects
Details
- Language :
- English
- ISSN :
- 0192415X
- Volume :
- 52
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- American Journal of Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 176812643
- Full Text :
- https://doi.org/10.1142/S0192415X24500186