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Inflammatory Cytokines and Chemokines Are Synergistically Induced in a ROS-Dependent Manner by a Co-Culture of Corneal Epithelial Cells and Neutrophil-like Cells in the Presence of Particulate Matter.
- Source :
- Antioxidants; Apr2024, Vol. 13 Issue 4, p467, 15p
- Publication Year :
- 2024
-
Abstract
- Ocular exposure to particulate matter (PM) causes local inflammation; however, the influence of neutrophils on PM-induced ocular inflammation is still not fully understood. In this study, we constructed a system to investigate the role of PM in ocular inflammation using a co-culture of human corneal epithelial cells (HCE-T) and differentiation-induced neutrophils (dHL-60). To investigate whether HCE-T directly endocytosed PM, we performed a holographic analysis, which showed the endocytosis of PM in HCE-T. The cytokines and chemokines produced by HCE-T were measured using an ELISA. HCE-T treated with PM produced IL-6 and IL-8, which were inhibited by N-Acetyl-L-cysteine (NAC), suggesting the involvement of ROS. Their co-culture with dHL-60 enhanced their production of IL-6, IL-8, and MCP-1. This suggests an inflammatory loop involving intraocular corneal epithelial cells and neutrophils. These cytokines and chemokines are mainly regulated by NF-κB. Therefore, this co-culture system was examined in the presence of an IKK inhibitor known to downregulate NF-κB activity. The IKK inhibitor dramatically suppressed the production of these factors in co-culture supernatants. The results suggest that the inflammatory loop observed in the co-culture is mediated through ROS and the transcription factor NF-κB. Thus, the co-culture system is considered a valuable tool for analyzing complex inflammations. [ABSTRACT FROM AUTHOR]
- Subjects :
- PARTICULATE matter
CHEMOKINES
CORNEA
EYE inflammation
CYTOKINES
CHEMOKINE receptors
Subjects
Details
- Language :
- English
- ISSN :
- 20763921
- Volume :
- 13
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Antioxidants
- Publication Type :
- Academic Journal
- Accession number :
- 176874710
- Full Text :
- https://doi.org/10.3390/antiox13040467