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Impact of gamma irradiation on phytochemical composition, and biological activities of Lepidium sativum seeds extract.

Authors :
Hadj Rabia, Samia
Debib, Aicha
Eddaikra, Atika
Aberkane-Mchebbek, Lila
Nouri, Ramy
Benmoussa, Fatima
Mokhtari, Amine
Medjber, Sarah
Mansouri, Belkacem
Messaoudi, Mohammed
Source :
Radiochimica Acta; May2024, Vol. 112 Issue 5, p351-362, 12p
Publication Year :
2024

Abstract

The main objective of this research is to assess how gamma radiation influences the chemical composition and activities such as antioxidant, antimicrobial and anticancer activities of Lepidium sativum (L. sativum) seeds. Methanolic extracts were obtained through a 24 h maceration process from L. sativum seeds exposed to various doses of gamma irradiation. Phytochemical screening involved precipitation and staining reactions, with the antioxidant potential assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Antimicrobial activity was evaluated through a diffusion test on agar medium, while the cytotoxic potential on the lung cancer line "A-549 cells" was examined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Qualitative analysis of the extracts identified preserved bioactive constituents after irradiation. Additionally, quantitative analysis indicated a significant rise in the total content of polyphenols and flavonoids under radiation, accompanied by an enhanced reducing power compared to the control. The antimicrobial and cytotoxic capabilities also improved, evident in increased growth inhibition zones for certain microbial strains and reduced viability rates of A549 cells. These results show promise and warrant further investigation into the potential of gamma radiation to enhance additional biological effects of plants. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00338230
Volume :
112
Issue :
5
Database :
Complementary Index
Journal :
Radiochimica Acta
Publication Type :
Academic Journal
Accession number :
177041719
Full Text :
https://doi.org/10.1515/ract-2023-0260