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Dissecting the immune discrepancies in mouse liver allograft tolerance and heart/kidney allograft rejection.
- Source :
- Cell Proliferation; Mar2024, Vol. 57 Issue 3, p1-15, 15p
- Publication Year :
- 2024
-
Abstract
- The liver is the most tolerogenic of transplanted organs. However, the mechanisms underlying liver transplant tolerance are not well understood. The comparison between liver transplantation tolerance and heart/kidney transplantation rejection will deepen our understanding of tolerance and rejection in solid organs. Here, we built a mouse model of liver, heart and kidney allograft and performed single-cell RNA sequencing of 66,393 cells to describe the cell composition and immune cell interactions at the early stage of tolerance or rejection. We also performed bulk RNA-seq of mouse liver allografts from Day 7 to Day 60 posttransplantation to map the dynamic transcriptional variation in spontaneous tolerance. The transcriptome of lymphocytes and myeloid cells were characterized and compared in three types of organ allografts. Cell-cell interaction networks reveal the coordinated function of Kupffer cells, macrophages and their associated metabolic processes, including insulin receptor signalling and oxidative phosphorylation in tolerance induction. Cd11b+ dendritic cells (DCs) in liver allografts were found to inhibit cytotoxic T cells by secreting anti-inflammatory cytokines such as Il10. In summary, we profiled single-cell transcriptome analysis of mouse solid organ allografts. We characterized the immune microenvironment of mouse organ allografts in the acute rejection state (heart, kidney) and tolerance state (liver). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09607722
- Volume :
- 57
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Cell Proliferation
- Publication Type :
- Academic Journal
- Accession number :
- 177054215
- Full Text :
- https://doi.org/10.1111/cpr.13555