Back to Search
Start Over
Identification of individual plants carrying viruses or a viroid in Pyrus betulifolia seedlings and virus-tested pear mother plants by group testing.
- Source :
- Journal of Plant Diseases & Protection; Jun2024, Vol. 131 Issue 3, p971-976, 6p
- Publication Year :
- 2024
-
Abstract
- Monitoring the sanitary status of a large population of vegetative propagation materials is a challenge because it requires expensive and time-consuming testing. Here we report a group testing strategy to identify individual plants carrying viruses and/or a viroid in Pyrus betulifolia seedlings and virus-tested pear mother plants. First, the effect of pool size on RT-PCR detection of apple stem grooving virus (ASGV), apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV) and apple scar skin viroid (ASSVd) was evaluated; all of them could be sensitively detected in a bulk (pool) of eight samples. The virological condition of 2770 P. betulifolia seedlings and 110 virus-tested pear mother plants were then investigated by RT-PCR using a grouping strategy with 5 samples. We identified 20, 35 and 3 P. betulifolia seedlings as positive for ASGV, ASPV, and ASSVd, respectively, and 4 pear mother plants were identified as carrying ASGV. Our data indicated that RT-PCR testing on samples pooled before RNA extraction is a reliable and resource-efficient strategy to monitor the sanitary status of large numbers of pear vegetative propagation materials. The results presented here also highlight the possibility of pear seedling rootstocks being a source of viral infection. [ABSTRACT FROM AUTHOR]
- Subjects :
- PLANT viruses
PLANT identification
PEARS
VEGETATIVE propagation
SEEDLINGS
Subjects
Details
- Language :
- English
- ISSN :
- 18613829
- Volume :
- 131
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of Plant Diseases & Protection
- Publication Type :
- Academic Journal
- Accession number :
- 177194378
- Full Text :
- https://doi.org/10.1007/s41348-023-00850-5