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Porcine humoral immune responses to multiple injections of murine monoclonal antibodies.
- Source :
- APMIS; Jul/Aug2005, Vol. 113 Issue 7/8, p489-496, 8p, 1 Chart, 1 Graph
- Publication Year :
- 2005
-
Abstract
- Lohse L, Nielsen J, Kamstrup S, Oleksiewicz MB, Eriksen L. Porcine humoral immune responses to multiple injections of murine monoclonal antibodies. APMIS 2005;113:489–96. In humans and cattle, multiple injections of murine monoclonal antibodies (m-mAbs) induce anti-mouse antibody responses. The objectives of the present study were to investigate whether a similar response could be seen when pigs were subjected to m-mAb therapy, and to study the kinetics of such a response. In two separate animal experiments, long-term treatment was performed with m-mAbs at low-dose levels and therapeutic levels, respectively. Two specific m-mAbs that recognized cognate antigen in the pigs (CD4 and CD8 surface antigens on T-lymphocytes) and two irrelevant control m-mAbs having no cognate antigen in the pigs were used. Enzyme-linked immunosorbent assays (ELISA) were used to quantitate the circulating m-mAbs, as well as the induced pig anti-mouse antibodies (PAMA), in serum samples from m-mAb-treated pigs. As expected, we generally saw vigorous PAMA responses within 10 days after the start of m-mAb treatment with the specific m-mAbs. However, the different mAbs showed striking differences in the kinetics and levels of PAMA responses, differences that might be ascribed to the m-mAb formulation and epitope specificity. In conclusion, treatment of pigs with m-mAbs against T-cell surface antigens induced rapid PAMA responses. This may influence and possibly decrease the effect of the m-mAb treatment by narrowing the time period where m-mAbs can efficiently be used for cell depletion. [ABSTRACT FROM AUTHOR]
- Subjects :
- MONOCLONAL antibodies
T cells
LYMPHOCYTES
THERAPEUTICS
ANTIGENS
CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 09034641
- Volume :
- 113
- Issue :
- 7/8
- Database :
- Complementary Index
- Journal :
- APMIS
- Publication Type :
- Academic Journal
- Accession number :
- 17742830
- Full Text :
- https://doi.org/10.1111/j.1600-0463.2005.apm_62.x