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Genomic Characterization of Carbapenemase-Producing Enterobacter hormaechei , Serratia marcescens , Citrobacter freundii , Providencia stuartii , and Morganella morganii Clinical Isolates from Bulgaria.
- Source :
- Antibiotics (2079-6382); May2024, Vol. 13 Issue 5, p455, 13p
- Publication Year :
- 2024
-
Abstract
- Carbapenemase-producing Enterobacter spp. Serratia marcescens, Citrobacter freundii, Providencia spp., and Morganella morganii (CP-ESCPM) are increasingly identified as causative agents of nosocomial infections but are still not under systematic genomic surveillance. In this study, using a combination of whole-genome sequencing and conjugation experiments, we sought to elucidate the genomic characteristics and transferability of resistance genes in clinical CP-ESCPM isolates from Bulgaria. Among the 36 sequenced isolates, NDM-1 (12/36), VIM-4 (11/36), VIM-86 (8/36), and OXA-48 (7/36) carbapenemases were identified; two isolates carried both NDM-1 and VIM-86. The majority of carbapenemase genes were found on self-conjugative plasmids. IncL plasmids were responsible for the spread of OXA-48 among E. hormaechei, C. freundii, and S. marcescens. IncM2 plasmids were generally associated with the spread of NDM-1 in C. freundii and S. marcescens, and also of VIM-4 in C. freundii. IncC plasmids were involved in the spread of the recently described VIM-86 in P. stuartii isolates. IncC plasmids carrying bla<subscript>NDM-1</subscript> and bla<subscript>VIM-86</subscript> were observed too. bla<subscript>NDM-1</subscript> was also detected on IncX3 in S. marcescens and on IncT plasmid in M. morganii. The significant resistance transfer rates we observed highlight the role of the ESCPM group as a reservoir of resistance determinants and stress the need for strengthening infection control measures. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20796382
- Volume :
- 13
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Antibiotics (2079-6382)
- Publication Type :
- Academic Journal
- Accession number :
- 177459749
- Full Text :
- https://doi.org/10.3390/antibiotics13050455