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A Review of the Rumen Microbiota and the Different Molecular Techniques Used to Identify Microorganisms Found in the Rumen Fluid of Ruminants.

Authors :
Silva, Éder Bruno Rebelo da
Silva, Jamile Andréa Rodrigues da
Silva, Welligton Conceição da
Belo, Tatiane Silva
Sousa, Carlos Eduardo Lima
Santos, Maria Roseane Pereira dos
Neves, Kedson Alessandri Lobo
Rodrigues, Thomaz Cyro Guimarães de Carvalho
Camargo-Júnior, Raimundo Nonato Colares
Lourenço-Júnior, José de Brito
Source :
Animals (2076-2615); May2024, Vol. 14 Issue 10, p1448, 20p
Publication Year :
2024

Abstract

Simple Summary: Environmental factors like climate, diet, and farming methods significantly affect microbial activity and composition. Understanding these adjustments is crucial for improving ruminant nutrition and production. This review summarizes research on rumen microbiota and molecular methods for microbial identification in ruminant rumen fluid. Analyzing rumen microbiology in various ruminants is challenging due to environmental and nutritional influences on microbial interactions. Key genera include Entodinium spp. and Aspergillus spp. for protozoa and fungi, respectively, with Fibrobacter spp. prevalent among bacteria. Major techniques involve DNA extraction, amplification, and sequencing. In conclusion, this review highlights the knowledge in the literature about rumen microorganisms and the associated molecular approaches. Variations in environments, including climate, diet, and agricultural practices, significantly impact the composition and microbial activity. A profound understanding of these adaptations allows for the improvement of nutrition and ruminant production. Therefore, this review aims to compile data from the literature on the rumen microbiota and molecular techniques for identifying the different types of microorganisms from the rumen fluid of ruminants. Analyzing the literature on rumen microbiology in different ruminants is complex due to microbial interactions, influenced by the environment and nutrition of these animals. In addition, it is worth noting that the genera of protozoa and fungi most evident in the studies used in this review on the microbiology of rumen fluid were Entodinium spp. and Aspergillus spp., respectively, and Fibrobacter spp. for bacteria. About the techniques used, it can be seen that DNA extraction, amplification, and sequencing were the most cited in the studies evaluated. Therefore, this review describes what is present in the literature and provides an overview of the main microbial agents in the rumen and the molecular techniques used. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20762615
Volume :
14
Issue :
10
Database :
Complementary Index
Journal :
Animals (2076-2615)
Publication Type :
Academic Journal
Accession number :
177459857
Full Text :
https://doi.org/10.3390/ani14101448