PEG-Based Clotrimzole Microparticles Improved Ischemia-Reperfusion Injury in Rat Testicular Torsion and Detorsion Model: Evaluation of Tissue Oxidative Stress.

A rat testis model was used to assess effects of polyethylene glycol (PEG) clotrimazole microparticles on ischemia-reperfusion injury. Forty-eight healthy male Wistar rats were included and randomized into six investigational groups (n = 8): Group SHAM: Merely laparotomy was implemented. Group ISCHEMIA: Merely a 3-hour interval ischemia was done. Group I/R: A 3-hour interval ischemia, three-hour reperfusion for left testis, and one-week reperfusion for right testis were done and 20 µL normal saline was administered intraperitoneally (IP) 30 min before termination of ischemia. Group I/R/PEG: The same as group I/R as well as 20 µL PEG (IP) 30 min before termination of ischemia. Group I/R/CLTMZLMP: The same as group I/R and 20 µl (10 mg/kg) of clotrimazole microparticles (IP) 30 min before termination of ischemia. Group I/R/PEGCLTMZLMP: The same as group I/R and 20 µL PEG-based clotrimazole microparticles (IP) 30 min before termination of ischemia. Evaluations were based on analyses of biochemical assays. PEG-based clotrimazole microparticles improved enhanced antioxidant activity (p < 0.05). PEG-based clotrimazole microparticles could be helpful in minimizing ischemia-reperfusion injury in testicular tissue exposed to ischemia [ABSTRACT FROM AUTHOR]