Live-cell imaging and CLEM reveal the existence of ACTN4-dependent ruffle-edge lamellipodia acting as a novel mode of cell migration.

A preprint abstract from biorxiv.org discusses the correlation between the expression levels of ACTN4 and the invasive and metastatic potential of cancer cells. The study used live-cell imaging and correlative light and electron microscopy (CLEM) to identify ACTN4-localized ruffle-edge lamellipodia, which were found to be most abundant in the leading edges of lamellipodia. The study also found that overexpression of ACTN4 promoted lamellipodial formation and that ACTN4-enriched lamellipodia were highly dynamic and associated with cell migration. The research suggests that ruffle-edge lamellipodia may contribute to active cell migration and invasion in certain cancer cell types. However, it is important to note that this preprint has not been peer-reviewed. [Extracted from the article]