Biodegradation of Steroid Hormone Estriol by Pseudomonas proteolytica GBPI_Hb61, a Psychrotolerant Himalayan Bacteria.

The Pseudomonasproteolytica GBPI_Hb61 had shown estriol degradation potential. The conditions such as pH, temperature, inoculum volume, agitation speed, and compound concentration were optimized for designing the experiments for getting the maximum degradation using the Box-Behnken design. Bacteria were able to degrade 85.23% of 20 mg L⁻¹ estriol when conditions were media pH 7.0, 25 °C temperature, 6% (v/v) inoculum volume, and 130 rpm agitation speed. Degradation intermediates such as 16-hydroxyestrone, 2-hydroxy2-4-dienevaleric acid, and 2-hydroxy-2,4-diene-1,6-dioic acid were identified through metabolite fragment analysis, and enzyme investigation has shown the presence of acetaldehyde dehydrogenase, dioxygenase, and acetyl COA acetyltransferase in GBPI_Hb61 which are reported to be responsible for estrogen degradation in the environment. GBPI_Hb61 was also found capable of removing estriol in soil. [ABSTRACT FROM AUTHOR]