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miR‐148a‐3p regulates proliferation and apoptosis of idiopathic gingival fibroma by targeting NPTX1.
- Source :
- Oral Diseases; May2024, Vol. 30 Issue 4, p2136-2149, 14p
- Publication Year :
- 2024
-
Abstract
- Objective: Idiopathic gingival fibromatosis (IGF) is a rare heterogeneous disease that results in the progressive and diffuse hyperplasia of gingival tissues. MicroRNAs are implicated in the development and progression of various tumors. The present study aimed to explore the potential roles and mechanisms of miR‐148a‐3p in IGF. Methods: Gingival fibroblasts (GFs) were transfected with miR‐148a‐3p mimics, miR‐148a‐3p inhibitors, or siNPTX1, and then, the proliferation and apoptosis of GFs and the expression of related genes were evaluated using Cell Counting Kit‐8 assays, 5‐ethynyl‐2′‐deoxyuridine assays, flow cytometry, reverse transcription‐quantitative polymerase chain reaction, and western blot analysis, respectively. Results: miR‐148a‐3p was highly expressed in GFs of IGF (IGF‐GFs) as compared with normal GFs (N‐GFs). Overexpression of miR‐148a‐3p promoted the proliferation and inhibited the apoptosis of N‐GFs, whereas downregulation of miR‐148a‐3p had the opposite effect in IGF‐GFs. Knockdown of NPTX1 reversed miR‐148a‐3p‐mediated effects in IGF‐GFs. Dual‐luciferase reporter assay confirmed that NPTX1 is a direct target of miR‐148a‐3p. Conclusion: These findings identify that miR‐148a‐3p could regulate cell proliferation and apoptosis by targeting NPTX1, providing new insights for the further study of the molecular mechanism and treatment of IGF. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROTEINS
HYPERPLASIA
FLOW cytometry
COMPUTER-assisted molecular modeling
RESEARCH funding
T-test (Statistics)
MICRORNA
CELL proliferation
APOPTOSIS
GINGIVA
REVERSE transcriptase polymerase chain reaction
CELL cycle
DESCRIPTIVE statistics
FIBROBLASTS
CYTOMETRY
WESTERN immunoblotting
GENE expression profiling
ANALYSIS of variance
GINGIVAL hyperplasia
TUMORS
DATA analysis software
COMPARATIVE studies
ORAL health
DISEASE progression
SEQUENCE analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1354523X
- Volume :
- 30
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Oral Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 177613944
- Full Text :
- https://doi.org/10.1111/odi.14655