Third Military Medical University - Army Medical University Researchers Update Knowledge of Biosensors (Multiarmed DNA jumper and metal-organic frameworks-functionalized paper-based bioplatform for small extracellular vesicle-derived miRNAs...).

Researchers from Third Military Medical University - Army Medical University have developed a novel biosensor for the detection of small extracellular vesicle-derived microRNAs (sEV-miRNAs), which are promising biomarkers for early cancer diagnosis. The biosensor utilizes a molecular probe based on multiarmed DNA tetrahedral jumpers (mDNA-Js) and a disposable paper-based electrode modified with a Zr-MOF-rGO-Au NP nanocomplex. The bioplatform achieved sensitive detection of sEV-miR-21 with a detection limit of 34.6 aM and a dynamic range from 100 aM to 0.2 M. The biosensor shows potential for fast, easy, low-cost, and highly sensitive detection of various nucleic acids, making it suitable for point-of-care biosensing. [Extracted from the article]