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The GA and ABA signaling is required for hydrogen-mediated seed germination in wax gourd.

Authors :
Chang, Jingjing
Li, Jiawei
Li, Jinlong
Chen, Xiao
Jiao, Jiabin
Li, Jing
Song, Zhao
Zhang, Baige
Source :
BMC Plant Biology; 6/13/2024, Vol. 24 Issue 1, p1-15, 15p
Publication Year :
2024

Abstract

Background: Hydrogen gas (H<subscript>2</subscript>), a novel and beneficial gaseous molecule, plays a significant role in plant growth and development processes. Hydrogen-rich water (HRW) is regarded as a safe and easily available way to study the physiological effects of H<subscript>2</subscript> on plants. Several recent research has shown that HRW attenuates stress-induced seed germination inhibition; however, the underlying modes of HRW on seed germination remain obscure under non-stress condition. Results: In this current study, we investigated the possible roles of gibberellin (GA) and abscisic acid (ABA) in HRW-regulated seed germination in wax gourd (Benincasa hispida) through pharmacological, physiological, and transcriptome approaches. The results showed that HRW application at an optimal dose (50% HRW) significantly promoted seed germination and shortened the average germination time (AGT). Subsequent results suggested that 50% HRW treatment stimulated GA production by regulating GA biosynthesis genes (BhiGA3ox, BhiGA2ox, and BhiKAO), whereas it had no effect on the content of ABA and the expression of its biosynthesis (BhiNCED6) and catabolism genes (BhiCYP707A2) but decreased the expression of ABA receptor gene (BhiPYL). In addition, inhibition of GA production by paclobutrazol (PAC) could block the HRW-mediated germination. Treatment with ABA could hinder HRW-mediated seed germination and the ABA biosynthesis inhibitor sodium tungstate (ST) could recover the function of HRW. Furthermore, RNA-seq analysis revealed that, in the presence of GA or ABA, an abundance of genes involved in GA, ABA, and ethylene signal sensing and transduction might involve in HRW-regulated germination. Conclusions: This study portrays insights into the mechanism of HRW-mediated seed germination, suggesting that HRW can regulate the balance between GA and ABA to mediate seed germination through ethylene signals in wax gourd. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14712229
Volume :
24
Issue :
1
Database :
Complementary Index
Journal :
BMC Plant Biology
Publication Type :
Academic Journal
Accession number :
177880227
Full Text :
https://doi.org/10.1186/s12870-024-05193-3