A novel UV-resistant bacterium Sphingomonas endolithica sp. nov., and genomic analysis, isolated from the north slope of Mount Everest.

Gram-stain-negative, aerobic, rod-shaped, non-motile bacterium strain ZFBP2030^T was isolated from a rock on the North slope of Mount Everest. This strain contained a unique ubiquinone-10 (Q-10) as a predominant respiratory quinone. Among the tested fatty acids, the strain contained summed feature 8, C_14:0 2OH, and C_16:0, as major cellular fatty acids. The polar lipid profile contained phosphatidyl glycerol, phosphatidyl ethanolamine, three unidentified phospholipids, two unidentified aminolipids, and six unidentified lipids. The cell-wall peptidoglycan was a meso-diaminopimelic acid, and cell-wall sugars were ribose and galactose. Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain ZFBP2030^T was a member of the genus Sphingomonas, exhibiting high sequence similarity to the 16S rRNA gene sequences of Sphingomonas aliaeris DH-S5^T (97.9%), Sphingomonas alpina DSM 22537^T (97.3%) and Sphingomonas hylomeconis CCTCC AB 2013304^T (97.0%). The 16S rRNA gene sequence similarity between ZFBP2030^T and other typical strains was less than 97.0%. The average amino acid identity values, average nucleotide identity, and digital DNA–DNA hybridization values between strain ZFBP2030^T and its highest sequence similarity strains were 56.9–79.9%, 65.1–82.2%, and 19.3–25.8%, respectively. The whole-genome size of the novel strain ZFBP2030^T was 4.1 Mbp, annotated with 3838 protein-coding genes and 54 RNA genes. Moreover, DNA G + C content was 64.7 mol%. Stress-related functions predicted in the subsystem classification of the strain ZFBP2030^T genome included osmotic, oxidative, cold/heat shock, detoxification, and periplasmic stress responses. The overall results of this study clearly showed that strain ZFBP2030^T is a novel species of the genus Sphingomonas, for which the name Sphingomonas endolithica sp. nov. is proposed. The type of strain is ZFBP2030^T (= EE 013^T = GDMCC 1.3123^T = JCM 35386^T). [ABSTRACT FROM AUTHOR]