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Prostate cancer reshapes the secreted and extracellular vesicle urinary proteomes.

Authors :
Khoo, Amanda
Govindarajan, Meinusha
Qiu, Zhuyu
Liu, Lydia Y.
Ignatchenko, Vladimir
Waas, Matthew
Macklin, Andrew
Keszei, Alexander
Neu, Sarah
Main, Brian P.
Yang, Lifang
Lance, Raymond S.
Downes, Michelle R.
Semmes, O. John
Vesprini, Danny
Liu, Stanley K.
Nyalwidhe, Julius O.
Boutros, Paul C.
Kislinger, Thomas
Source :
Nature Communications; 6/13/2024, Vol. 15 Issue 1, p1-16, 16p
Publication Year :
2024

Abstract

Urine is a complex biofluid that reflects both overall physiologic state and the state of the genitourinary tissues through which it passes. It contains both secreted proteins and proteins encapsulated in tissue-derived extracellular vesicles (EVs). To understand the population variability and clinical utility of urine, we quantified the secreted and EV proteomes from 190 men, including a subset with prostate cancer. We demonstrate that a simple protocol enriches prostatic proteins in urine. Secreted and EV proteins arise from different subcellular compartments. Urinary EVs are faithful surrogates of tissue proteomes, but secreted proteins in urine or cell line EVs are not. The urinary proteome is longitudinally stable over several years. It can accurately and non-invasively distinguish malignant from benign prostatic lesions and can risk-stratify prostate tumors. This resource quantifies the complexity of the urinary proteome and reveals the synergistic value of secreted and EV proteomes for translational and biomarker studies. Urine can be used to easily analyse the health of patients. Here, the authors identify proteins in urinary extracellular vesicles that distinguish prostate cancer from benign lesions. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20411723
Volume :
15
Issue :
1
Database :
Complementary Index
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
177898148
Full Text :
https://doi.org/10.1038/s41467-024-49424-5