Patent Application Titled "Programmable Nucleases And Methods Of Use" Published Online (USPTO 20240191224).

The US Patent and Trademark Office has published a patent application titled "Programmable Nucleases and Methods of Use." The application, filed by inventors Janice Sha Chen, Lucas Benjamin Harrington, and Isaac Paterson Witte, and assigned to Mammoth Biosciences Inc., explains a technique for introducing breaks in target nucleic acids using programmable nickases. These enzymes have the potential for genome editing and diagnostics. The application provides extensive information on the structure and characteristics of the programmable nickases, including their length, sequence identity, and the types of proteins they are. The methods described in the application can be used to modify genes associated with diseases like facioscapulohumeral muscular dystrophy and cancer. Additionally, the application outlines a method for detecting target nucleic acids in a sample using a programmable nickase and a labeled, single-stranded DNA reporter. For more details and specific claims, readers are encouraged to refer to the full document. [Extracted from the article]