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Disruption of the cell division protein ftsK gene changes elemental selenium generation, selenite tolerance, and cell morphology in Rahnella aquatilis HX2.
- Source :
- Journal of Applied Microbiology; Jun2024, Vol. 135 Issue 6, p1-13, 13p
- Publication Year :
- 2024
-
Abstract
- Aims Some studies have indicated that the alterations in cellular morphology induced by selenite [Se(Ⅳ)] may be attributed to its inhibitory effects on cell division. However, whether the genes associated with cell division are implicated in Se(Ⅳ) metabolism remains unclear. Methods and Results The fts K gene in Rahnella aquatilis HX2 was mutated with an in-frame deletion strategy. The fts K mutation strongly reduced the tolerance to selenite [Se(Ⅳ)] and the production of red elemental selenium [Se(0)] in R. aquatilis HX2, and this effect could not be attributed solely to the inhibition of cell growth. Deleting the fts K gene also resulted in a significant decrease in bacterial growth of R. aquatilis HX2 during both exponential and stationary phases. The deletion of fts K inhibited cell division, resulting in the development of elongated filamentous cells. Furthermore, the loss-of-function of FtsK significantly impacted the expression of seven genes linked to cell division and Se(Ⅳ) metabolism by at least 2-fold, as unveiled by real-time quantitative PCR (RT-qPCR) under Se(Ⅳ) treatment. Conclusions These findings suggest that FtsK is associated with Se(Ⅳ) tolerance and Se(0) generation and is a key player in coordinating bacterial growth and cell morphology in R. aquatilis HX2. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL morphology
CELL division
KILLER cells
SELENIUM
CELL growth
Subjects
Details
- Language :
- English
- ISSN :
- 13645072
- Volume :
- 135
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Journal of Applied Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 178159297
- Full Text :
- https://doi.org/10.1093/jambio/lxae142