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[ 68 Ga]FAPI PET for Imaging and Treatment Monitoring in a Preclinical Model of Pulmonary Fibrosis: Comparison to [ 18 F]FDG PET and CT.
- Source :
- Pharmaceuticals (14248247); Jun2024, Vol. 17 Issue 6, p726, 14p
- Publication Year :
- 2024
-
Abstract
- Purpose: This study aimed to evaluate the feasibility of using [<superscript>68</superscript>Ga]-fibroblast-activating protein inhibitor (FAPI) positron emission tomography (PET) imaging for diagnosing pulmonary fibrosis in a mouse model. We also examined its value in monitoring treatment response and compared it with traditional [<superscript>18</superscript>F]-fluorodeoxyglucose (FDG) PET and computed tomography (CT) imaging. Methods: A model of idiopathic pulmonary fibrosis was established using intratracheal injection of bleomycin (BLM, 2 mg/kg) into C57BL/6 male mice. For the treatment of IPF, a daily oral dose of 400 mg/kg/day of pirfenidone was administered from 9 to 28 days after the establishment of the model. Disease progression and treatment efficacy were assessed at different stages of the disease every week for four weeks using CT, [<superscript>18</superscript>F]FDG PET, and [<superscript>68</superscript>Ga]FAPI PET (baseline imaging performed at week 0). Mice were sacrificed and lung tissues were harvested for hematoxylin-eosin staining, picrosirius red staining, and immunohistochemical staining for glucose transporter 1 (GLUT1) and FAP. Expression levels of GLUT1 and FAP in pathological sections were quantified. Correlations between imaging parameters and pathological quantitative values were analyzed. Results: CT, [<superscript>18</superscript>F]FDG PET and [<superscript>68</superscript>Ga]FAPI PET revealed anatomical and functional changes in the lung that reflected progression of pulmonary fibrosis. In untreated mice with pulmonary fibrosis, lung uptake of [<superscript>18</superscript>F]FDG peaked on day 14, while [<superscript>68</superscript>Ga]FAPI uptake and mean lung density peaked on day 21. In mice treated with pirfenidone, mean lung density and lung uptake of both PET tracers decreased. Mean lung density, [<superscript>18</superscript>F]FDG uptake, and [<superscript>68</superscript>Ga]FAPI uptake correlated well with quantitative values of picrosirius red staining, GLUT1 expression, and FAP expression, respectively. Conclusions: Although traditional CT and [<superscript>18</superscript>F]FDG PET reflect anatomical and metabolic status in fibrotic lung, [<superscript>68</superscript>Ga]FAPI PET provides a means of evaluating fibrosis progression and monitoring treatment response. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14248247
- Volume :
- 17
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Pharmaceuticals (14248247)
- Publication Type :
- Academic Journal
- Accession number :
- 178193535
- Full Text :
- https://doi.org/10.3390/ph17060726