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Genetic variability of human papillomavirus type 18 based on E6, E7 and L1 genes in central China.
- Source :
- Virology Journal; 7/5/2024, Vol. 21 Issue 1, p1-12, 12p
- Publication Year :
- 2024
-
Abstract
- Background: High-risk human papillomavirus (HR-HPV) infection is an important factor for the development of cervical cancer. HPV18 is the second most common HR-HPV after HPV16. Methods: In this study, MEGA11 software was used to analyze the variation and phylogenetic tree of HPV18 E6-E7 and L1 genes. The selective pressure to E6, E7 and L1 genes was estimated using pamlX. In addition, the B cell epitopes of L1 amino acid sequences and T cell epitopes of E6-E7 amino acid sequences in HPV18 were predicted by ABCpred server and IEDB website, respectively. Results: A total of 9 single nucleotide variants were found in E6-E7 sequences, of which 2 were nonsynonymous variants and 7 were synonymous variants. Twenty single nucleotide variants were identified in L1 sequence, including 11 nonsynonymous variants and 9 synonymous variants. Phylogenetic analysis showed that E6-E7 and L1 sequences were all distributed in A lineage. In HPV18 E6, E7 and L1 sequences, no positively selected site was found. The nonconservative substitution R545C in L1 affected hypothetical B cell epitope. Two nonconservative substitutions, S82A in E6, and R53Q in E7, impacted multiple hypothetical T cell epitopes. Conclusion: The sequence variation data of HPV18 may lay a foundation for the virus diagnosis, further study of cervical cancer and vaccine design in central China. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1743422X
- Volume :
- 21
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Virology Journal
- Publication Type :
- Academic Journal
- Accession number :
- 178295803
- Full Text :
- https://doi.org/10.1186/s12985-024-02424-9