Efficacy of thymol trimethoxycinnamate on melanin production, transfer and AGEs synthesis.

The whitening effect of thymol trimethoxycinnamate was studied based on skin cell model. CCK-8 method and cell state observation were used to detect the effect of thymol trimethoxycinnamate on the viability of B16-F10 cells. NaOH lysis method and dopa oxidation method were used to analyze the changes of melanin content and tyrosinase activity in B16-F10 cells. Cell scratch method and Transwell chamber method were used to determine the migration ability of B16-F10 cells. Fluorescence analysis was used to detect the changes of fluorescent AGEs content in the saccharification experiment in vitro. The results show that thymol trimethoxycinnamate has no significant effect on cell viability at or below 10 µg/mL, and has the highest inhibition rate on melanin synthesis, tyrosinase activity and cell migration at 10 µg/mL. The inhibition rate of fluorescent AGEs production in vitro is 97.75% at 200 µg/mL. The results show that thymol trimethoxycinnamate can obviously inhibit the formation of melanin and tyrosinase activity, and has a good whitening effect. Because of its good anti-saccharification ability, it can be used as a potential anti-aging material. [ABSTRACT FROM AUTHOR]